Antioxidants activities of four different solvent extracts of Corchorus tridens leaf using DPPH free radical scavenging assay

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Original research (Published On: 23-Jan-2023 )

Antioxidants activities of four different solvent extracts of Corchorus tridens leaf using DPPH free radical scavenging assay

O. A. Ushie, A. C. Kendeson, B. D. Longbab and A. Galadima

J. Chem. Res. Adv., 04 (01):10-14

O. A. Ushie : Department of Chemical Science, Federal University Wukari, Nigeria

A. C. Kendeson : Department of Chemical Sciences, Federal University of Kashere, Nigeria

B. D. Longbab : Department of Chemical Science, Federal University Wukari, Nigeria

A. Galadima : Department of Chemical Science, Federal University Wukari, Nigeria

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Article History: Received on: 22-Nov-22, Accepted on: 15-Jan-23, Published on: 23-Jan-23

Corresponding Author: O. A. Ushie

Email: ushie@fuwukari.edu.ng

Citation: Ushie OA, Kendeson AC, Longbab BD and Galadima A (2023). Antioxidants activities of four different solvent extracts of Corchorus tridens leaf using DPPH free radical scavenging assay. J. Chem. Res. Adv., 04 (01 ):10-14

Abstract

Aim: This study was aimed to evaluate and compare antioxidant activities of four crude extracts of C. tridens leaves which locally used as a delicacy in many parts of Nigeria especially the South-western part.

Method and Materials: The samples of leaves of C. tridens plant were collected from the environment. A 100 g powdered sample of plucked leaves after proper treatment was weighed and transferred into extraction container for serial exhaustive extraction using cold maceration method for 96 hours (4 days) using 250 mL each of hexane, ethyl acetate, acetone, and methanol. At the end of each extraction, the extract was filtered using filter paper and evaporated to dryness on a water bath at 60 °C to obtain a thick sticky, dark colored extract. The percentage yields were calculated and noted. The antioxidant activities of the crude extracts of C. tridens leaf was determined using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method.

Results: The free radical scavenging potential of the crude extracts and the standard (Vitamin C) as indicated by the maximal inhibitory concentration (IC₅₀) values showed that methanol extract had the highest inhibitory effect (34.38 mg/mL) of all the crude extracts, followed by acetone extract (37.31 mg/mL), ethyl acetate extract (46.69 mg/mL) and the least was hexane (66.38 mg/mL) as compared to the standard (Vitamin C) which had a value of (22.52 mg/mL).

Conclusion: It was concluded that all the extracts exhibited antioxidant activity but methanol extract had the most antioxidant activity which was comparable with the standard used. This may be due to phyto-compounds in the solvent owing to its polarity, hence, plant’s leaves can be used to manage oxidative stress.

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