Ahmad Shoujaa, Nawaf Mouzaffar, Bassel Hussein, Wael Al-Aghawani and Antoun AL-laham
J. Chem. Res. Adv., 03 (02):07-13
Ahmad Shoujaa : Dr
Nawaf Mouzaffar : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria
Bassel Hussein : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria
Wael Al-Aghawani : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria
Antoun AL-laham : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria
Aim: This study was aimed to evaluate simultaneous and rapid separation and determination of vitamins b1, b2, pp, b6 by using ion-pair rp-hplc and ultra violet detection in multivitamin tablets.
Method and Materials: The HPLC system (shimadzu-japan) consist of control unit (scl-10-A vp) , oven(CTO-10A vp) and two types of detectors: Ultra violet Detector (SPD-10AV vp), fluorescent Detector (RF-10AXL), degassing unit (DGU-14A) isocratic pump, with valve 20-µL loop, (LC-solution) software was used for collected data, Chromatographic analysis was performed on RP-C18 (250 mm x4. 6 mm, 5 µm particle size) column.
Results: Simultaneous determination of B1, B2, B3, B6 vitamins were obtained with above mobile phase where the pH was adjusted to 5 ± 0.2 by using 2N sodium hydroxide solution to prevent peaks interfering during separation the mixture of studied compounds, mobile phase was filtered through 0. 45μm Millipore filter, flow rate 1ml/min and octadecyle column ODS C18 (250x4. 6,5µm), measurements were made at λ=280 nm..
Conclusion: It was concluded that simultaneous determination of the four water-soluble vitamins thiamine hydrochloride, riboflavin, nicotinamide and pyridoxine hydrochloride was performed on column of c18(250x4. 6,5µm), a mixture of ( water: methanol: glacial acetic acid) ratio(72:27:1 v/v) as the mobile phase with flow rate of 1mL/men, the effluent was monitored at 280 nm.