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Original research (Published On: 01-Nov-2022 )

Simultaneous and rapid separation and determination of vitamins b1, b2, pp, b6 by using ion-pair rp-hplc and ultra violet detection in multivitamin tablets

Ahmad Shoujaa, Nawaf Mouzaffar, Bassel Hussein, Wael Al-Aghawani and Antoun AL-laham

J. Chem. Res. Adv., 03 (02):07-13

Ahmad Shoujaa : Dr

Nawaf Mouzaffar : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria

Bassel Hussein : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria

Wael Al-Aghawani : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria

Antoun AL-laham : Faculty of Pharmacy, Al-Sham Private University (ASPU), Damascus, Syria

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Article History: Received on: 23-Jun-22, Accepted on: 08-Oct-22, Published on: 01-Nov-22

Corresponding Author: Ahmad Shoujaa

Email: a.s.foph@aspu.edu.sy

Citation: Mouzaffar N, Hussein B, Al-Aghawani W, Shoujaa A and AL-laham A (2022). Simultaneous and rapid separation and determination of vitamins b1, b2, pp, b6 by using ion-pair rp-hplc and ultra violet detection in multivitamin tablets. J. Chem. Res. Adv., 03 (02 ):07-13


Abstract

Aim: This study was aimed to evaluate simultaneous and rapid separation and determination of vitamins b1, b2, pp, b6 by using ion-pair rp-hplc and ultra violet detection in multivitamin tablets.

Method and Materials: The HPLC system (shimadzu-japan) consist of control unit  (scl-10-A vp) , oven(CTO-10A vp) and two types of detectors:  Ultra violet Detector (SPD-10AV vp), fluorescent Detector   (RF-10AXL), degassing  unit (DGU-14A)  isocratic pump, with valve 20-µL loop, (LC-solution)  software  was used for collected data,  Chromatographic analysis was performed on RP-C18 (250 mm x4. 6 mm, 5 µm particle size) column.

Results: Simultaneous determination of B1, B2, B3, B6 vitamins were obtained with above mobile phase where the pH was adjusted to 5 ± 0.2 by using 2N sodium hydroxide solution to prevent peaks interfering during separation the mixture of studied compounds, mobile phase was filtered through 0. 45μm Millipore filter, flow rate 1ml/min and octadecyle column ODS C18 (250x4. 6,5µm), measurements were made at λ=280 nm..

Conclusion: It was concluded that simultaneous determination of the four water-soluble vitamins thiamine hydrochloride, riboflavin, nicotinamide and pyridoxine hydrochloride was performed on column of c18(250x4. 6,5µm), a mixture  of ( water: methanol: glacial acetic acid) ratio(72:27:1 v/v) as the mobile phase with flow rate of 1mL/men, the effluent was monitored at 280 nm.


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